| Gene | Tmie |
| Chromosome | 9 |
| Deletion Start | 110,873,888 |
| Deletion End | 110,866,795 |
| Deletion Size | 7,094 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,873,663 | 110,873,683 | CCTTGCCCTTCACGCTTAGAG |
| TUP | 110,873,639 | 110,873,660 | ACTGGTCTGACGGCGGAGCACA |
| TUR | 110,873,609 | 110,873,632 | CAAACTCCTTGTGTCCCTGAAGTC |
| TDF | 110,867,376 | 110,867,397 | CACTTCCTCACTCCCACTGTTC |
| TDP | 110,867,349 | 110,867,372 | TTGAACCTGTGTCTGCCCTCTCGC |
| TDR | 110,867,301 | 110,867,321 | TTTCTGCACTCTGCTCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTTGAGTGGAATGGGAGG |
| SD | TGCTATTTGAGGCGGCTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13905A-F11 | VGB6 | C57BL/6NTac | |
| 13905A-G8 | VGB6 | C57BL/6NTac | |
| 13905A-C9 | VGB6 | C57BL/6NTac | |
