| Gene | Tmsb4x |
| Chromosome | X |
| Deletion Start | 167,208,033 |
| Deletion End | 167,207,480 |
| Deletion Size | 554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,208,010 | 167,208,031 | TGACAAACCCGATATGGCTGAG |
| TUP | 167,207,974 | 167,207,998 | CGATAAGTCGAAGTTGAAGAAAACA |
| TUR | 167,207,922 | 167,207,944 | GGGCGAACAGCTTACTTTCTTTT |
| TDF | 167,207,646 | 167,207,669 | GTACTTCTGTTTAGCATGTCTTGC |
| TDP | 167,207,626 | 167,207,645 | TTAGCGAGGCTGCTATGTGT |
| TDR | 167,207,489 | 167,207,509 | GCGCTCGCCTCATTACGATTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAATGGCTTCGATCTATCC |
| SD | ACTCCTTCCAGTCACCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 181 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13906A-A8 | VGB6 | C57BL/6NTac | |
| 13906A-B4 | VGB6 | C57BL/6NTac | |
| 13906A-B8 | VGB6 | C57BL/6NTac | |
| 13906A-D2 | VGB6 | C57BL/6NTac | |
| 13906A-G12 | VGB6 | C57BL/6NTac | |
