| Gene | Toe1 |
| Chromosome | 4 |
| Deletion Start | 116,807,516 |
| Deletion End | 116,804,434 |
| Deletion Size | 3,083 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,807,358 | 116,807,379 | AGTTCTTCTTGCCTCAGCTATC |
| TUP | 116,807,330 | 116,807,353 | ACTACAACTCCCAGTGTGCAGCGC |
| TUR | 116,807,286 | 116,807,307 | CCTCACTGCTCCACTCTACAAC |
| TDF | 116,804,744 | 116,804,764 | AGCTTGGAGATGGCACATAGG |
| TDP | 116,804,714 | 116,804,740 | ACAAGTGCTGAAACAGCTGATGTGGCC |
| TDR | 116,804,676 | 116,804,696 | GGGTTAGTGCTGGCTTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTTAAGATTCCACGTTCCCG |
| SD | CTGGAGCTTTGGGAACAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13907A-C10 | VGB6 | C57BL/6NTac | |
| 13907A-D3 | VGB6 | C57BL/6NTac | |
| 13907A-E2 | VGB6 | C57BL/6NTac | |
| 13907A-G10 | VGB6 | C57BL/6NTac | |
| 13907A-G9 | VGB6 | C57BL/6NTac | |
| 13907A-E7 | VGB6 | C57BL/6NTac | |
