| Gene | Tppp3 |
| Chromosome | 8 |
| Deletion Start | 105,468,624 |
| Deletion End | 105,467,740 |
| Deletion Size | 885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,468,534 | 105,468,554 | CCAGCGGGCAAGAGATGAATG |
| TUP | 105,468,509 | 105,468,532 | CAAGAACTGGGCCAAGCTGTGCAA |
| TUR | 105,468,489 | 105,468,507 | CGTCGGCCACCTTACAGTC |
| TDF | 105,467,998 | 105,468,016 | AACGGGTGGTGCTGTGGAC |
| TDP | 105,467,970 | 105,467,997 | CGGCTGACGGACACCAGTAAGTATACGG |
| TDR | 105,467,929 | 105,467,949 | CTTTCCCTTGCCGCTCTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGGACAGTGCCCTTCTGTTC |
| SD | GAAGAGCCTAGCAGGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13909A-A9 | VGB6 | C57BL/6NTac | |
| 13909A-H11 | VGB6 | C57BL/6NTac | |
| 13909A-E1 | VGB6 | C57BL/6NTac | |
| 13909A-E9 | VGB6 | C57BL/6NTac | |
| 13909A-F2 | VGB6 | C57BL/6NTac | |
