| Gene | Traf6 |
| Chromosome | 2 |
| Deletion Start | 101,684,504 |
| Deletion End | 101,697,597 |
| Deletion Size | 13,094 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,684,591 | 101,684,612 | GTGAAAGATGACAGCGTGAGTG |
| TUP | 101,684,614 | 101,684,636 | CTCTGCCAGCACCGGGAACCTCT |
| TUR | 101,684,677 | 101,684,697 | CTCTCCAGAGGTGGGTCAAAC |
| TDF | 101,696,642 | 101,696,662 | ACGGCTTGTGCTTCATTTCAG |
| TDP | 101,696,664 | 101,696,688 | TGCAGAGGAATCACTTGGCACGACA |
| TDR | 101,696,696 | 101,696,717 | TCATGTGCAACTGGGTATTCTC |
PCR Primers
| Primer | Sequence |
| SU | TAAACAGATGACAGAGGAAGG |
| SD | AGAACTCAACACCAGAGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13911A-B6 | VGB6 | C57BL/6NTac | |
| 13911A-H5 | VGB6 | C57BL/6NTac | |
| 13911A-F3 | VGB6 | C57BL/6NTac | |
| 13911A-D2 | VGB6 | C57BL/6NTac | |
