| Gene | Trp63 |
| Chromosome | 16 |
| Deletion Start | 25,802,063 |
| Deletion End | 25,820,638 |
| Deletion Size | 18,576 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,802,918 | 25,802,937 | GAGGGCTCTGAGGATGTGTC |
| TUP | 25,802,939 | 25,802,961 | TAAGGCTGGCCGGCAAGGTCTAA |
| TUR | 25,803,004 | 25,803,025 | CCCTCCCAATCAGATCAGTGTC |
| TDF | 25,820,416 | 25,820,438 | ACAGCATGGACCAGCAGATTCAG |
| TDP | 25,820,439 | 25,820,460 | AACGGCTCCTCGTCCACCAGCC |
| TDR | 25,820,465 | 25,820,483 | CTGTGCGTGGTCTGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCTTCTGTGTGTCTGGTG |
| SD | TGGAACCTGTCTATATTCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13920A-B8 | VGB6 | C57BL/6NTac | |
| 13920A-E6 | VGB6 | C57BL/6NTac | |
| 13920A-E9 | VGB6 | C57BL/6NTac | |
| 13920A-G9 | VGB6 | C57BL/6NTac | |
