| Gene | Trpt1 |
| Chromosome | 19 |
| Deletion Start | 6,996,468 |
| Deletion End | 6,999,046 |
| Deletion Size | 2,579 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,996,652 | 6,996,670 | CAGGGCTGGCTACTGAAAC |
| TUP | 6,996,671 | 6,996,700 | TCAGATCATCACTGGACTCTTTCAGGACCG |
| TUR | 6,996,725 | 6,996,743 | GTGGCGAAGGGCATAGGAC |
| TDF | 6,998,166 | 6,998,185 | CTGCCCGTTCCCACACTTAC |
| TDP | 6,998,187 | 6,998,210 | TGCAGGTCCCTGAGTTGGAACTGA |
| TDR | 6,998,259 | 6,998,277 | GGCCAGTGCTTCCAGAATG |
PCR Primers
| Primer | Sequence |
| SU | TTGATCTCGGAATTTAGCGG |
| SD | AGCTTACAGGAGGCCATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13922A-F9 | VGB6 | C57BL/6NTac | |
| 13922A-H2 | VGB6 | C57BL/6NTac | |
| 13922A-H12 | VGB6 | C57BL/6NTac | |
| 13922A-H6 | VGB6 | C57BL/6NTac | |
