| Gene | Trub2 |
| Chromosome | 2 |
| Deletion Start | 29,787,608 |
| Deletion End | 29,777,625 |
| Deletion Size | 9,984 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,787,495 | 29,787,516 | GCAACTGCTTAAAGGTGAGAAC |
| TUP | 29,787,474 | 29,787,493 | CCGACGCGGACCCACGGTTT |
| TUR | 29,787,411 | 29,787,432 | AAACAGCTACAACCCGGCAAAC |
| TDF | 29,778,351 | 29,778,372 | GTTCCTTAGCAGTTCCACCTTG |
| TDP | 29,778,317 | 29,778,342 | CCCTCAGGCATGACTGTCACTTCCTG |
| TDR | 29,778,273 | 29,778,292 | CCCACGGAAACAGGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TAACCGAGAATTCGGTTGCTC |
| SD | TCTGTGAAAAGATCATCGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13923A-D1 | VGB6 | C57BL/6NTac | |
| 13923A-E3 | VGB6 | C57BL/6NTac | |
| 13923A-A1 | VGB6 | C57BL/6NTac | |
