| Gene | Tspan1 |
| Chromosome | 4 |
| Deletion Start | 116,167,061 |
| Deletion End | 116,162,885 |
| Deletion Size | 4,177 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,166,932 | 116,166,950 | AACAGAGGCCAGGCTGGAG |
| TUP | 116,166,903 | 116,166,929 | TGGGAAACTAGGTACCCAGACTCTGCC |
| TUR | 116,166,845 | 116,166,868 | TCAGGAATCAGCATTTGGGAAGAC |
| TDF | 116,163,372 | 116,163,395 | CCAAGGGTTGTTTCAAAGAGATTC |
| TDP | 116,163,339 | 116,163,366 | AGAATCAGAGCCAATGCAGTCACTGTGG |
| TDR | 116,163,317 | 116,163,335 | GCAACTCCAACTGCCACAC |
PCR Primers
| Primer | Sequence |
| SU | GCAGTTTCCTGGAGCTGTTC |
| SD | TTAGGCAATCAGGACCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13925A-A10 | VGB6 | C57BL/6NTac | |
| 13925A-B10 | VGB6 | C57BL/6NTac | |
| 13925A-C8 | VGB6 | C57BL/6NTac | |
| 13925A-F7 | VGB6 | C57BL/6NTac | |
| 13925A-F5 | VGB6 | C57BL/6NTac | |
| 13925A-D2 | VGB6 | C57BL/6NTac | |
