| Gene | Tspan31 |
| Chromosome | 10 |
| Deletion Start | 127,070,174 |
| Deletion End | 127,067,813 |
| Deletion Size | 2,362 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,069,656 | 127,069,673 | TTGGGCTGTGCTGCATTG |
| TUP | 127,069,629 | 127,069,653 | AGAAATCTTTGGCGATGACCGGGTC |
| TUR | 127,069,581 | 127,069,601 | CAGCGAAGTTCCAGGTTAGTC |
| TDF | 127,068,517 | 127,068,538 | GCTGTGGCTTGTTCAACCTTAC |
| TDP | 127,068,487 | 127,068,514 | CCCTGCGCCTACAAGATGATACTTCCTG |
| TDR | 127,068,438 | 127,068,461 | GCTGTAGTGAATGTCCCAGGAATG |
PCR Primers
| Primer | Sequence |
| SU | CTGGTCTCTAAGACACTGTC |
| SD | ACTCTGTCCTTGAAGCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13926A-D6 | VGB6 | C57BL/6NTac | |
| 13926A-F8 | VGB6 | C57BL/6NTac | |
| 13926A-H4 | VGB6 | C57BL/6NTac | |
