| Gene | Tssk5 |
| Chromosome | 15 |
| Deletion Start | 76,374,681 |
| Deletion End | 76,372,040 |
| Deletion Size | 2,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,373,817 | 76,373,841 | TCAACTGTATGAGACCTACCAGAAC |
| TUP | 76,373,792 | 76,373,816 | AGCCAGCGCTCCTACCTGGTACTGG |
| TUR | 76,373,773 | 76,373,790 | TCACCTCTAGCTGCCAAC |
| TDF | 76,372,158 | 76,372,176 | TCCAGTGGCAGGACATCTC |
| TDP | 76,372,136 | 76,372,157 | CAAGGAGACCGTCTCTGGCCCA |
| TDR | 76,372,078 | 76,372,098 | CAAGCCCAAGGGTGGTAGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACACCCAGTCCTTTGGAGTC |
| SD | GTCTCCAAGGGAGTTTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13927A-B8 | VGB6 | C57BL/6NTac | |
| 13927A-C10 | VGB6 | C57BL/6NTac | |
| 13927A-D11 | VGB6 | C57BL/6NTac | |
| 13927A-E4 | VGB6 | C57BL/6NTac | |
| 13927A-H6 | VGB6 | C57BL/6NTac | |
| 13927A-D9 | VGB6 | C57BL/6NTac | |
