| Gene | Tst |
| Chromosome | 15 |
| Deletion Start | 78,405,830 |
| Deletion End | 78,399,694 |
| Deletion Size | 6,137 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,405,684 | 78,405,704 | GCACGCAAAGAGTACCAGGAG |
| TUP | 78,405,658 | 78,405,679 | ACGTGCCTGGCGCGTCCTTCTT |
| TUR | 78,405,568 | 78,405,587 | CCCAGGTTGCCCACATAGTC |
| TDF | 78,399,835 | 78,399,853 | CCCTGGCTGCCTACCTTTG |
| TDP | 78,399,810 | 78,399,834 | TGGCAAACCCGATGTGGCTGTTTAC |
| TDR | 78,399,780 | 78,399,798 | CCTGCGGAACCACTCTGAC |
PCR Primers
| Primer | Sequence |
| SU | AGACAGAGTCCTGAGCCAGC |
| SD | TCTGTAGGAAATGTTTCAATTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13928A-E4 | VGB6 | C57BL/6NTac | |
| 13928A-H5 | VGB6 | C57BL/6NTac | |
