| Gene | Tsta3 |
| Chromosome | 15 |
| Deletion Start | 75,929,034 |
| Deletion End | 75,924,866 |
| Deletion Size | 4,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,928,703 | 75,928,724 | TCCTCCTGTAGTTCCGGGATTC |
| TUP | 75,928,680 | 75,928,702 | CCAGCCGACAGCTGCTAAAGCTC |
| TUR | 75,928,648 | 75,928,667 | AGGGCAGCCACAGAGACAAG |
| TDF | 75,925,006 | 75,925,026 | AGTTACTGGCTCTCCCTTGTC |
| TDP | 75,924,981 | 75,925,005 | TGTCTCTGCAGCTGTGAAGGAAACC |
| TDR | 75,924,946 | 75,924,965 | CGGGCCTGCTCATAGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GGACTCCAAGTACGAGCACC |
| SD | CTGCTGCCTGACCTCCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13929A-A11 | VGB6 | C57BL/6NTac | |
| 13929A-F7 | VGB6 | C57BL/6NTac | |
| 13929A-C7 | VGB6 | C57BL/6NTac | |
| 13929A-D11 | VGB6 | C57BL/6NTac | |
| 13929A-E4 | VGB6 | C57BL/6NTac | |
| 13929A-D2 | VGB6 | C57BL/6NTac | |
