| Gene | Tubb2b |
| Chromosome | 13 |
| Deletion Start | 34,130,230 |
| Deletion End | 34,127,396 |
| Deletion Size | 2,835 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,130,079 | 34,130,100 | CGCGGCTCTAGGTAAATACCAG |
| TUP | 34,130,056 | 34,130,077 | ACTCACCAACGGCGGACCTGAC |
| TUR | 34,130,018 | 34,130,036 | CTCTGAGACCACGCCCATC |
| TDF | 34,128,200 | 34,128,224 | CACAGATGAAACCTACTGCATCGAT |
| TDP | 34,075,163 | 34,075,188 | TCTGTATGACATCTGTTTCCGCACCC |
| TDR | 34,075,117 | 34,075,137 | GACACCAGGTGGTTGAGATCG |
PCR Primers
| Primer | Sequence |
| SU | GGCTCAGAGCATTACGTCAAC |
| SD | TTTCTGTGTTCAGAAAGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 53108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13931A-H10 | VGB6 | C57BL/6NTac | |
| 13931A-B11 | VGB6 | C57BL/6NTac | |
| 13931A-C10 | VGB6 | C57BL/6NTac | |
| 13931A-D3 | VGB6 | C57BL/6NTac | |
| 13931A-H9 | VGB6 | C57BL/6NTac | |
| 13931A-F5 | VGB6 | C57BL/6NTac | |
