| Gene | Tufm |
| Chromosome | 7 |
| Deletion Start | 126,487,451 |
| Deletion End | 126,490,625 |
| Deletion Size | 3,175 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,487,852 | 126,487,872 | CGTAGGGTGGGTGTCTTGTAG |
| TUP | 126,487,874 | 126,487,898 | CCGCTGAACTTTAGCATGGCTCCAA |
| TUR | 126,487,901 | 126,487,918 | GCCTTCGTCCCAAGTGTC |
| TDF | 126,490,261 | 126,490,280 | CAGCGGTTGAAGGTACACAG |
| TDP | 126,490,281 | 126,490,305 | TGAAGGTATCCTGATTGCCTTGCGC |
| TDR | 126,490,321 | 126,490,339 | CTCCAGGCATGGCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TAATGGCCAAGGACTGGAAC |
| SD | ACAATGGCTTTGAGCCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13932A-G7 | VGB6 | C57BL/6NTac | |
| 13932A-G10 | VGB6 | C57BL/6NTac | |
