| Gene | Nprl2 |
| Chromosome | 9 |
| Deletion Start | 107,542,379 |
| Deletion End | 107,545,600 |
| Deletion Size | 3,222 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,543,139 | 107,543,156 | GGCAGGCTGACTCTGTTC |
| TUP | 107,543,157 | 107,543,179 | TACCTCTCCAGCACAGCCATGGA |
| TUR | 107,543,211 | 107,543,231 | GCGGCTGTACTTCTTGTGTTC |
| TDF | 107,544,779 | 107,544,800 | CCAGGATCTGGTGGATGACAAG |
| TDP | 107,544,804 | 107,544,828 | CTGCAGGAGGCATGTCTGTCCTACG |
| TDR | 107,544,841 | 107,544,859 | CGCCAGGGACTGCCATTAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTCACCTGCTTCTCAGG |
| SD | TTTGCCCACTAGATGGCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13933A-G12 | VGB6 | C57BL/6NTac | |
| 13933B-H6 | VGB6 | C57BL/6NTac | |
| 13933B-H12 | VGB6 | C57BL/6NTac | |
