| Gene | Twist2 |
| Chromosome | 1 |
| Deletion Start | 91,801,630 |
| Deletion End | 91,802,154 |
| Deletion Size | 525 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,801,664 | 91,801,683 | ACAGTCTGGGCACCAGTGAG |
| TUP | 91,801,685 | 91,801,706 | AAGAGCTGGAGAGGCAGCCCAA |
| TUR | 91,801,734 | 91,801,755 | GCCATCTTCGCTCGATTTCTTG |
| TDF | 91,801,992 | 91,802,016 | GAGCGACGAGATGGACAATAAGATG |
| TDP | 91,802,022 | 91,802,044 | CTGCAGCTACGTGGCTCACGAGC |
| TDR | 91,802,058 | 91,802,076 | CATGCGCCACACGGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGACAGCTATTTAGCAACC |
| SD | GGATCAGAAAGAGCGGATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13934A-B2 | VGB6 | C57BL/6NTac | |
| 13934A-C8 | VGB6 | C57BL/6NTac | |
| 13934A-C9 | VGB6 | C57BL/6NTac | |
| 13934A-G4 | VGB6 | C57BL/6NTac | |
| 13934A-D11 | VGB6 | C57BL/6NTac | |
| 13934A-D8 | VGB6 | C57BL/6NTac | |
