| Gene | Wbp1 |
| Chromosome | 6 |
| Deletion Start | 83,121,268 |
| Deletion End | 83,119,167 |
| Deletion Size | 2,102 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,120,824 | 83,120,845 | CAATTTGGAGACGAGCTGGAAC |
| TUP | 83,120,801 | 83,120,822 | CAGACTCGCGCCCTGGATACCA |
| TUR | 83,120,772 | 83,120,792 | CAGATGGGATTGCTGTGGATG |
| TDF | 83,119,442 | 83,119,461 | CCGCTATCGTCGCCTAACTG |
| TDP | 83,119,416 | 83,119,440 | TGACTCGGGTATTGAGCTCTGCCCT |
| TDR | 83,119,367 | 83,119,386 | CACTAGCCCTCGCTTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCAGTCAGGCATTTGGTC |
| SD | GGCTTGGGTTTCTGGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13941A-B10 | VGB6 | C57BL/6NTac | |
| 13941A-B4 | VGB6 | C57BL/6NTac | |
