| Gene | Wdr38 |
| Chromosome | 2 |
| Deletion Start | 38,998,396 |
| Deletion End | 39,001,403 |
| Deletion Size | 3,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,999,163 | 38,999,184 | GGAGTGGGACCTGGAGTTTATG |
| TUP | 38,999,187 | 38,999,207 | CCTACCCGGGCTTCCTGGACA |
| TUR | 38,999,233 | 38,999,254 | TGCCTGTCTGGAGTCATTTGTC |
| TDF | 39,000,623 | 39,000,641 | GGTTCCTGGGACTCGACTG |
| TDP | 39,000,642 | 39,000,665 | TGCACATATGGGACTTGCGGGCAT |
| TDR | 39,000,686 | 39,000,704 | TGTGGCCCTCCAGATTGTG |
PCR Primers
| Primer | Sequence |
| SU | CAGAGGAGCTCTGGATCTGG |
| SD | CTTCAGCATCTTCCCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13942A-H11 | VGB6 | C57BL/6NTac | |
| 13942A-D8 | VGB6 | C57BL/6NTac | |
| 13942A-F12 | VGB6 | C57BL/6NTac | |
| 13942A-E9 | VGB6 | C57BL/6NTac | |
| 13942A-B7 | VGB6 | C57BL/6NTac | |
