| Gene | Wnt1 |
| Chromosome | 15 |
| Deletion Start | 98,790,192 |
| Deletion End | 98,792,942 |
| Deletion Size | 2,751 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,791,094 | 98,791,113 | TCTTCGGCAAGATCGTCAAC |
| TUP | 98,791,123 | 98,791,143 | TGCCCAGGAAAGCGACGCTTC |
| TUR | 98,791,167 | 98,791,185 | CGCCTATGCCCTGGAGATG |
| TDF | 98,792,652 | 98,792,675 | CGACCTCGTCTACTTCGAGAAATC |
| TDP | 98,792,677 | 98,792,701 | CCCAACTTCTGCACGTACAGTGGCC |
| TDR | 98,792,763 | 98,792,782 | CACAGCACAGCAGCTCACAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGCCACAGCTTCGCTC |
| SD | CCCAGAGGTGCTGGTTAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 131 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13944A-C4 | VGB6 | C57BL/6NTac | |
| 13944A-G4 | VGB6 | C57BL/6NTac | |
| 13944A-A4 | VGB6 | C57BL/6NTac | |
| 13944A-H7 | VGB6 | C57BL/6NTac | |
