| Gene | Yipf2 |
| Chromosome | 9 |
| Deletion Start | 21,592,555 |
| Deletion End | 21,589,643 |
| Deletion Size | 2,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,592,145 | 21,592,164 | GCCAAAGTGATGAGCTGACC |
| TUP | 21,592,125 | 21,592,144 | TCCCGAGAGCACGTGGCTGT |
| TUR | 21,592,104 | 21,592,123 | CCAATGCCTGAACCCACAAC |
| TDF | 21,589,886 | 21,589,905 | GCTGCGGCACTATTGTCTAC |
| TDP | 21,589,865 | 21,589,885 | TGTGGTGCTGCTTCACGCCCT |
| TDR | 21,589,814 | 21,589,832 | CACAGCCCTGGTGAGAATC |
PCR Primers
| Primer | Sequence |
| SU | ACGGACCTAAACGGATGTTG |
| SD | GGCTCTCTCCTGTCCATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13946A-D2 | VGB6 | C57BL/6NTac | |
| 13946A-E11 | VGB6 | C57BL/6NTac | |
| 13946A-E2 | VGB6 | C57BL/6NTac | |
| 13946A-E10 | VGB6 | C57BL/6NTac | |
| 13946A-E7 | VGB6 | C57BL/6NTac | |
| 13946A-G6 | VGB6 | C57BL/6NTac | |
