| Gene | Ypel4 |
| Chromosome | 2 |
| Deletion Start | 84,736,828 |
| Deletion End | 84,737,822 |
| Deletion Size | 995 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,736,868 | 84,736,888 | CCAAGACTTTCCGCAGTTACC |
| TUP | 84,736,893 | 84,736,912 | CCGCTGCCACCGCACTTACA |
| TUR | 84,736,921 | 84,736,940 | GCCAAGTGTGCTCGACAGTG |
| TDF | 84,737,418 | 84,737,437 | AGGGTCAACGTGGGTTGTGG |
| TDP | 84,737,440 | 84,737,462 | CAGCTGAACAGCGCCTCCTGCTC |
| TDR | 84,737,496 | 84,737,516 | CAGTGTGGTCTTGCAGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAAGGTACCAAATCCCTGGC |
| SD | CCACCACCAATGTTTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13948A-B5 | VGB6 | C57BL/6NTac | |
| 13948A-B9 | VGB6 | C57BL/6NTac | |
| 13948A-D1 | VGB6 | C57BL/6NTac | |
| 13948A-H7 | VGB6 | C57BL/6NTac | |
| 13948A-D3 | VGB6 | C57BL/6NTac | |
| 13948A-D11 | VGB6 | C57BL/6NTac | |
