Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13951 Zdhhc12 Status: ES cell colonies screened / QC positives

Gene	Zdhhc12
Chromosome	2
Deletion Start	30,093,585
Deletion End	30,091,159
Deletion Size	2,427
Genome Build	38

MGI ID	MGI:1913470
NCBI Gene ID	66220
BAC ID	319K9
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	30,093,086	30,093,109	CCCAAATCCTATGAGCCGACTAAC
TUP	30,093,060	30,093,082	CCCAAGGGACGCCACTATAGCGA
TUR	30,093,034	30,093,052	CCAGGCGAAGGTGGGAAAC
TDF	30,092,045	30,092,063	CGAGCCCAAGGAGGAACAG
TDP	30,092,021	30,092,043	CAGCCATGGTTCCTCAGGCCGTC
TDR	30,091,983	30,092,002	CCTACCAGCACCAGACAGTG

PCR Primers

Primer	Sequence
SU	ATCAAAGATGGGCACGCATG
SD	AGCCTCCACGTAACCATGTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	76 bp	+	+	-
TDF	TDR	81 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13951A-A2	VGB6	C57BL/6NTac
13951A-D8	VGB6	C57BL/6NTac
13951A-H6	VGB6	C57BL/6NTac
13951A-D11	VGB6	C57BL/6NTac