| Gene | Zfp113 |
| Chromosome | 5 |
| Deletion Start | 138,152,896 |
| Deletion End | 138,144,567 |
| Deletion Size | 8,330 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,152,325 | 138,152,347 | GGGAGCTACAGATAGGAGGATTC |
| TUP | 138,152,296 | 138,152,323 | CAGAAGTTCATAGGCCAGCTAGCATGGA |
| TUR | 138,152,232 | 138,152,252 | GTGTCAGTCCTTGCCTTCTAC |
| TDF | 138,145,193 | 138,145,216 | TGAGTGTAAGGATTGTGGGAAGAC |
| TDP | 138,145,163 | 138,145,190 | TCAGTTGTAGCTCTGCTCTCATTCTGCA |
| TDR | 138,145,073 | 138,145,093 | CTCTGGTGGTGAGTGAGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGTGGAGTGATAGCCACC |
| SD | AACCTGTCTGTCTTCTCGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 144 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13953A-A5 | VGB6 | C57BL/6NTac | |
| 13953A-A9 | VGB6 | C57BL/6NTac | |
