| Gene | Zfp770 |
| Chromosome | 2 |
| Deletion Start | 114,197,583 |
| Deletion End | 114,195,388 |
| Deletion Size | 2,196 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,197,140 | 114,197,161 | GGCACTACACCCATGCTCTAAG |
| TUP | 114,197,114 | 114,197,137 | TGATGCCACATACAGCACCACGAA |
| TUR | 114,197,017 | 114,197,037 | CCTCTGCCCGGTGTGAATAAG |
| TDF | 114,195,739 | 114,195,756 | TACCTCGCCAACGCACTG |
| TDP | 114,195,712 | 114,195,737 | TGGAGTCAGAGCAGAGCCATCATTGT |
| TDR | 114,195,672 | 114,195,692 | GGAGCCCGTCACTTCTTTCAG |
PCR Primers
| Primer | Sequence |
| SU | AATGACAAAGCAACTTATTGGC |
| SD | ATGTACGCTTCCATGTAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13967A-C8 | VGB6 | C57BL/6NTac | |
| 13967A-D9 | VGB6 | C57BL/6NTac | |
| 13967A-H2 | VGB6 | C57BL/6NTac | |
