| Gene | Znhit3 |
| Chromosome | 11 |
| Deletion Start | 84,916,338 |
| Deletion End | 84,911,466 |
| Deletion Size | 4,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,916,277 | 84,916,298 | TGGAGAAGCCGAAATACCGTTG |
| TUP | 84,916,252 | 84,916,272 | ACTTGCCGCGTGCCCTAGTGA |
| TUR | 84,916,231 | 84,916,249 | GAACCCACTACCCGCTCTG |
| TDF | 84,911,565 | 84,911,587 | CCTCGATCAGGGTGACAACAAAG |
| TDP | 84,911,537 | 84,911,561 | AGCTGATGCGAGCCTGTATGCAGGA |
| TDR | 84,911,481 | 84,911,501 | TCTGGGATGGTTCCACGATTC |
PCR Primers
| Primer | Sequence |
| SU | GGGCAGGGACATCATTTAC |
| SD | GCTACAAGTTTCCTCTTCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13972A-B4 | VGB6 | C57BL/6NTac | |
| 13972A-H10 | VGB6 | C57BL/6NTac | |
| 13972A-H7 | VGB6 | C57BL/6NTac | |
| 13972A-F5 | VGB6 | C57BL/6NTac | |
| 13972A-G11 | VGB6 | C57BL/6NTac | |
| 13972A-D8 | VGB6 | C57BL/6NTac | |
