| Gene | Chchd10 |
| Chromosome | 10 |
| Deletion Start | 75,935,967 |
| Deletion End | 75,937,691 |
| Deletion Size | 1,725 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,936,515 | 75,936,534 | CAATGAGGAGTGGCCCAATG |
| TUP | 75,936,538 | 75,936,560 | TCAGAGTTGCCTCAGACCCAGGA |
| TUR | 75,936,594 | 75,936,615 | GCTTTCCAGCGAGTGACAGTAG |
| TDF | 75,937,605 | 75,937,623 | GGCCTGCTCATCGCCTAAC |
| TDP | 75,937,624 | 75,937,649 | CCCTCACCGACAGCTTGATGGAAAGG |
| TDR | 75,937,661 | 75,937,685 | ACGATCCTTCCTTACTCCCAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | CCGTGCCTAACTAAGCCTTG |
| SD | ATGTTCCTGTGGGCAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13977A-B1 | VGB6 | C57BL/6NTac | |
| 13977A-D1 | VGB6 | C57BL/6NTac | |
| 13977A-G8 | VGB6 | C57BL/6NTac | |
| 13977A-F9 | VGB6 | C57BL/6NTac | |
| 13977A-E9 | VGB6 | C57BL/6NTac | |
