| Gene | Fam173a |
| Chromosome | 17 |
| Deletion Start | 25,792,211 |
| Deletion End | 25,790,522 |
| Deletion Size | 1,690 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,791,439 | 25,791,460 | CCGTCCAGCTATGGGATATGAG |
| TUP | 25,791,412 | 25,791,434 | ACCCGTGGCTTGTGGGACTTGCA |
| TUR | 25,791,370 | 25,791,389 | GCAGACACTCGCAGAACAAC |
| TDF | 25,790,998 | 25,791,017 | CATGCCCTCCAGCCTTTACC |
| TDP | 25,790,967 | 25,790,992 | TTTCTACAGGTGAGCCTCAGGGACTG |
| TDR | 25,790,935 | 25,790,953 | CACTGGGAGCCAGGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CCATTCCATGTGGTTGTCTG |
| SD | TGAGGAAGTTGGTGAATATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13982A-E5 | VGB6 | C57BL/6NTac | |
| 13982A-B10 | VGB6 | C57BL/6NTac | |
| 13982A-C7 | VGB6 | C57BL/6NTac | |
| 13982A-H6 | VGB6 | C57BL/6NTac | |
| 13982A-H11 | VGB6 | C57BL/6NTac | |
