| Gene | Jmjd8 |
| Chromosome | 17 |
| Deletion Start | 25,829,069 |
| Deletion End | 25,830,621 |
| Deletion Size | 1,553 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,829,490 | 25,829,508 | CTTCCTCAAGCCCGTCATC |
| TUP | 25,829,510 | 25,829,533 | TGCAAGGACTCACGGACAACTCGG |
| TUR | 25,829,548 | 25,829,566 | CAGAAGCAGTTGCCCACAG |
| TDF | 25,830,328 | 25,830,350 | CACCTGAGTTCCACCCTAACAAG |
| TDP | 25,830,351 | 25,830,372 | ACCACATTGGCCTGGCTGCTGG |
| TDR | 25,830,382 | 25,830,402 | GTGCTGACAGGGCTAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | GGATGGAAGGTGGCTACAAC |
| SD | CTCTGACCCAGGAACAGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13985A-H9 | VGB6 | C57BL/6NTac | |
| 13985A-A5 | VGB6 | C57BL/6NTac | |
| 13985A-C12 | VGB6 | C57BL/6NTac | |
| 13985A-F7 | VGB6 | C57BL/6NTac | |
| 13985A-F4 | VGB6 | C57BL/6NTac | |
| 13985A-A2 | VGB6 | C57BL/6NTac | |
