| Gene | Kat5 |
| Chromosome | 19 |
| Deletion Start | 5,610,026 |
| Deletion End | 5,603,355 |
| Deletion Size | 6,672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,609,355 | 5,609,376 | GCAGTCAACAAACGTCTGGATG |
| TUP | 5,609,326 | 5,609,348 | TGACTCACGAGCGGCTGGACTTA |
| TUR | 5,609,275 | 5,609,296 | TCCGTTCTTGGTAGGTGTCTTG |
| TDF | 5,603,904 | 5,603,926 | GCCTCCTATCCTACCGAAGTTAC |
| TDP | 5,603,877 | 5,603,903 | TGGTCCCAAACCATCTTGGAGATCCTG |
| TDR | 5,603,832 | 5,603,852 | GATGGTGATCTGTGGCCTCTC |
PCR Primers
| Primer | Sequence |
| SU | GTGACACCTCACACTGCCAC |
| SD | ATGGCACCCAGTTTGGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13986A-H1 | VGB6 | C57BL/6NTac | |
| 13986B-F2 | VGB6 | C57BL/6NTac | |
| 13986C-C9 | VGB6 | C57BL/6NTac | |
