| Gene | Tmem219 |
| Chromosome | 7 |
| Deletion Start | 126,897,187 |
| Deletion End | 126,888,904 |
| Deletion Size | 8,284 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,896,837 | 126,896,855 | TCACGTCGTCGGCTTACTG |
| TUP | 126,896,808 | 126,896,835 | CCACTTTGAACTTCGGAGATGGTCCAGA |
| TUR | 126,896,770 | 126,896,794 | CATGGATCTTGGCTTGGAATGTTTG |
| TDF | 126,888,978 | 126,888,996 | GCCTCCTCTGCTGTACCAC |
| TDP | 126,888,952 | 126,888,974 | AGTCTGCTTCCATCCGCGCCCAG |
| TDR | 126,888,909 | 126,888,928 | GCAGTCTGTCGCCCTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAAACTGGAAACTGAACCC |
| SD | AAGGACACAGTTGAACAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13990A-C5 | VGB6 | C57BL/6NTac | |
| 13990A-D7 | VGB6 | C57BL/6NTac | |
| 13990A-D11 | VGB6 | C57BL/6NTac | |
| 13990A-E9 | VGB6 | C57BL/6NTac | |
| 13990A-E11 | VGB6 | C57BL/6NTac | |
| 13990A-E6 | VGB6 | C57BL/6NTac | |
