| Gene | Ydjc |
| Chromosome | 16 |
| Deletion Start | 17,146,986 |
| Deletion End | 17,148,530 |
| Deletion Size | 1,545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,147,300 | 17,147,319 | AACGCCTCGTCGCTCCTCAG |
| TUP | 17,147,321 | 17,147,345 | CCCGAAGGCTTCTTTCTCGGCAAGA |
| TUR | 17,147,348 | 17,147,368 | CGCTAAAGCCTCCCGAAATCC |
| TDF | 17,148,114 | 17,148,132 | CCGGTCCTTGAGGCAACAC |
| TDP | 17,148,134 | 17,148,160 | TCTGATATCCTGGCACTCACAGGTGGA |
| TDR | 17,148,168 | 17,148,189 | ACAAGTGCTCAGGCCCACAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATTTGCAGAGCGTTAGCAGG |
| SD | GCATGTGCAGAATTGGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13992A-A3 | VGB6 | C57BL/6NTac | |
| 13992A-A11 | VGB6 | C57BL/6NTac | |
| 13992A-B11 | VGB6 | C57BL/6NTac | |
| 13992A-C5 | VGB6 | C57BL/6NTac | |
| 13992A-G2 | VGB6 | C57BL/6NTac | |
| 13992A-G6 | VGB6 | C57BL/6NTac | |
