| Gene | 0610010K14Rik |
| Chromosome | 11 |
| Deletion Start | 70,237,801 |
| Deletion End | 70,235,397 |
| Deletion Size | 2,405 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,237,394 | 70,237,417 | GCTCAGTGTAGGTGGTAATTGTTG |
| TUP | 70,237,359 | 70,237,387 | TGGATGACAGGTAAAGAGACTACCGAGGC |
| TUR | 70,237,315 | 70,237,333 | GGGTAGGTGGCAGACATTC |
| TDF | 70,236,091 | 70,236,111 | GGTGTCCTCTCACCTCCAAAC |
| TDP | 70,236,066 | 70,236,087 | CCTCCACCCAGCAGCTCCAGTG |
| TDR | 70,236,004 | 70,236,023 | AGCAACTCCACAGCCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTGCTGTCTGTGCTTTCAG |
| SD | AATATTGGCTGTGCTGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13995A-H10 | VGB6 | C57BL/6NTac | |
| 13995A-B4 | VGB6 | C57BL/6NTac | |
| 13995A-H3 | VGB6 | C57BL/6NTac | |
| 13995A-E11 | VGB6 | C57BL/6NTac | |
| 13995A-E3 | VGB6 | C57BL/6NTac | |
| 13995A-D8 | VGB6 | C57BL/6NTac | |
