| Gene | 1110005A03Rik |
| Chromosome | 11 |
| Deletion Start | 116,843,694 |
| Deletion End | 116,849,594 |
| Deletion Size | 5,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,843,817 | 116,843,836 | GGGAGTGGGTTGTCCTTCAG |
| TUP | 116,843,837 | 116,843,861 | TTCTCGGCTTCGTTCATTAGCACCA |
| TUR | 116,843,863 | 116,843,881 | ACCGGGCTTCACACCAGAC |
| TDF | 116,848,779 | 116,848,801 | CCTTTAGTCCCAGCCTTCAGATG |
| TDP | 116,848,806 | 116,848,830 | CAGGCAGATGTTCAAAGCCAGCCTA |
| TDR | 116,848,888 | 116,848,910 | CAGCGCCAACCTAAATGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTTCATGCACGCCAATTGGG |
| SD | AAGGAAGCAAATGGTTTGTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13998A-B9 | VGB6 | C57BL/6NTac | |
| 13998A-C8 | VGB6 | C57BL/6NTac | |
