| Gene | Ecscr |
| Chromosome | 18 |
| Deletion Start | 35,721,407 |
| Deletion End | 35,713,366 |
| Deletion Size | 8,042 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,720,647 | 35,720,669 | CAGGTAGGGATTAGGAAGCAGAG |
| TUP | 35,720,618 | 35,720,645 | TCAGCAAAGTTAGGAGTTCTTGTGGCCA |
| TUR | 35,720,592 | 35,720,614 | GGGACATACAGACACCCAGAAAG |
| TDF | 35,713,821 | 35,713,843 | GTAGAGTTTTCTGGCATGAGGAC |
| TDP | 35,713,800 | 35,713,820 | TGAAGGCCCAGTGCTCCGTGG |
| TDR | 35,713,774 | 35,713,796 | GGATGGAGGACCAGGGAGTAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAAATGACTGGAATTCGACC |
| SD | AAAGAAATTGGATTCCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13999A-A4 | VGB6 | C57BL/6NTac | |
| 13999A-B3 | VGB6 | C57BL/6NTac | |
| 13999A-B8 | VGB6 | C57BL/6NTac | |
| 13999A-E12 | VGB6 | C57BL/6NTac | |
| 13999A-F1 | VGB6 | C57BL/6NTac | |
| 13999A-E7 | VGB6 | C57BL/6NTac | |
