| Gene | Ucma |
| Chromosome | 2 |
| Deletion Start | 4,976,255 |
| Deletion End | 4,985,502 |
| Deletion Size | 9,248 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,976,906 | 4,976,924 | GTAAGGCTGCTGGATGGTC |
| TUP | 4,976,925 | 4,976,947 | TCCCTCCCACTGTGGAAGACCAA |
| TUR | 4,976,965 | 4,976,985 | TCGTACTACTGCTCCCAGAAC |
| TDF | 4,985,099 | 4,985,122 | CAACCACCTATGACCTTTATCCTC |
| TDP | 4,985,123 | 4,985,145 | TGGCCTGCTCCAAGACAGAAACC |
| TDR | 4,985,187 | 4,985,208 | TGTACCTGAGCCATGAGACTTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCCCACTTGGACTCAGG |
| SD | CTTGGAGTTCTGTGGCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14000A-C11 | VGB6 | C57BL/6NTac | |
| 14000A-B4 | VGB6 | C57BL/6NTac | |
| 14000A-G7 | VGB6 | C57BL/6NTac | |
| 14000A-E8 | VGB6 | C57BL/6NTac | |
| 14000A-B6 | VGB6 | C57BL/6NTac | |
