| Gene | 1110032A03Rik |
| Chromosome | 9 |
| Deletion Start | 50,767,896 |
| Deletion End | 50,763,851 |
| Deletion Size | 4,046 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,767,810 | 50,767,834 | GAAGTAACATTGAACGCCTGTCTTG |
| TUP | 50,767,781 | 50,767,808 | TCCATAGCACTGTGAGAGCAACTAGGTG |
| TUR | 50,767,756 | 50,767,776 | GCAGGCCACTTCCAGTTCATC |
| TDF | 50,763,935 | 50,763,955 | CAAATGCACAGCGAAGTCAAC |
| TDP | 50,763,902 | 50,763,930 | ATGACTAACTACTCAGAACCTCAGCCCAC |
| TDR | 50,763,852 | 50,763,870 | GGCCCTAAGACTGGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | GATGACGCATCAACAGCTTG |
| SD | TGCTTTATTTAGACAGCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14001A-D8 | VGB6 | C57BL/6NTac | |
| 14001A-E8 | VGB6 | C57BL/6NTac | |
| 14001A-C10 | VGB6 | C57BL/6NTac | |
