| Gene | Leprel4 |
| Chromosome | 11 |
| Deletion Start | 100,414,653 |
| Deletion End | 100,408,689 |
| Deletion Size | 5,965 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,414,573 | 100,414,597 | CGAGAAATACAGCTTCCGAGGATTC |
| TUP | 100,414,549 | 100,414,570 | TCCGGAGGACCTGATGCCACTG |
| TUR | 100,414,509 | 100,414,529 | CGCCTTCGTATTGCTCCAGAG |
| TDF | 100,409,483 | 100,409,505 | GGAACTGGAGGAGACAGAATCAC |
| TDP | 100,409,455 | 100,409,482 | TCCCAGAGCCTGAAGAACCTCTATCTGA |
| TDR | 100,409,426 | 100,409,444 | CATAGTCGCCCTCGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | GCCAGTGGTGGTGTAAGCATTG |
| SD | CATAGGGTACACCAACTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14002A-B10 | VGB6 | C57BL/6NTac | |
| 14002A-H4 | VGB6 | C57BL/6NTac | |
| 14002A-C9 | VGB6 | C57BL/6NTac | |
| 14002A-E2 | VGB6 | C57BL/6NTac | |
| 14002A-D6 | VGB6 | C57BL/6NTac | |
| 14002A-C7 | VGB6 | C57BL/6NTac | |
