| Gene | 1110057K04Rik |
| Chromosome | 12 |
| Deletion Start | 8,220,605 |
| Deletion End | 8,238,610 |
| Deletion Size | 18,006 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,221,407 | 8,221,428 | CATGGGATCAGTAAGGCTGTTG |
| TUP | 8,221,429 | 8,221,452 | TTGCCCTCCTGGTCTTCAGGGATG |
| TUR | 8,221,495 | 8,221,512 | TGCAAGGAGGCAGAGGAC |
| TDF | 8,238,215 | 8,238,238 | TGAGTCGAGCTGTTTCAGATAGTG |
| TDP | 8,238,247 | 8,238,271 | CCAGTTCTGTGCGAAGGGCGAGTTA |
| TDR | 8,238,305 | 8,238,324 | TGCATCCCAGGCTTCTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCCAAGGGAACTGTCACTCC |
| SD | CAGAGTCCTTCCCATGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14003A-H3 | VGB6 | C57BL/6NTac | |
| 14003A-A7 | VGB6 | C57BL/6NTac | |
| 14003A-D9 | VGB6 | C57BL/6NTac | |
