| Gene | 1600002K03Rik |
| Chromosome | 10 |
| Deletion Start | 80,172,969 |
| Deletion End | 80,174,881 |
| Deletion Size | 1,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,173,100 | 80,173,118 | GGTGCTCCGCTTGTTCTAC |
| TUP | 80,173,120 | 80,173,141 | TAATCACTGGGCTCTGCGGCCT |
| TUR | 80,173,179 | 80,173,196 | CTGATTGGCCGCGCTAAC |
| TDF | 80,174,765 | 80,174,788 | CAACCAGTCCCATCCTTCAATACC |
| TDP | 80,174,791 | 80,174,817 | ATCTTGTCCTGTTCCTTCCCTTGCAGA |
| TDR | 80,174,822 | 80,174,842 | GCAGCCTGTCATCTCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCTGGACTGGACTTTCCC |
| SD | TGACATGAACAGGAGAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14007A-A12 | VGB6 | C57BL/6NTac | |
