| Gene | 1700007K13Rik |
| Chromosome | 2 |
| Deletion Start | 28,466,266 |
| Deletion End | 28,462,151 |
| Deletion Size | 4,116 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,465,661 | 28,465,679 | GGCCTCGGGTGTGAAGAAG |
| TUP | 28,465,634 | 28,465,658 | CGGCTGGTAGCCTCTATCACAGGCC |
| TUR | 28,465,591 | 28,465,612 | TGTCAGGAGGGAAGGAGGTAAG |
| TDF | 28,462,331 | 28,462,352 | CCCTGGAGAAGAGCTTAGTGAC |
| TDP | 28,462,307 | 28,462,330 | CGGGCCTGACAATCACATCACCCA |
| TDR | 28,462,258 | 28,462,276 | GGACGGCCTGTTGACATTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCAGGCAGAGATTGGAAG |
| SD | TGTGCACTTGACATTTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14008A-B6 | VGB6 | C57BL/6NTac | |
| 14008A-H8 | VGB6 | C57BL/6NTac | |
| 14008A-H2 | VGB6 | C57BL/6NTac | |
| 14008A-F6 | VGB6 | C57BL/6NTac | |
