| Gene | 1700019N12Rik |
| Chromosome | 19 |
| Deletion Start | 6,925,306 |
| Deletion End | 6,922,493 |
| Deletion Size | 2,814 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,925,086 | 6,925,104 | GGTGCCCAAACACGCAAAC |
| TUP | 6,925,063 | 6,925,085 | CATCGCCGCAGCTCGGTCAGATC |
| TUR | 6,925,029 | 6,925,049 | GCTCCATAGGTCACGAACATC |
| TDF | 6,922,823 | 6,922,844 | GCAACTTCTTCAGCCTTCTTCC |
| TDP | 6,922,796 | 6,922,822 | CCTCCATTCTTCACATCGCACCTAGGA |
| TDR | 6,922,754 | 6,922,777 | GTGCTGGATTTGGATCTGTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTATATCAGGCCCACTGC |
| SD | AAGAGTGGGCGTAGCTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14012D-G9 | VGB6 | C57BL/6NTac | |
| 14012D-C11 | VGB6 | C57BL/6NTac | |
| 14012D-G11 | VGB6 | C57BL/6NTac | |
| 14012D-G6 | VGB6 | C57BL/6NTac | |
