| Gene | 1700040I03Rik |
| Chromosome | 6 |
| Deletion Start | 85,451,176 |
| Deletion End | 85,447,082 |
| Deletion Size | 4,095 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,450,968 | 85,450,986 | AGCGGGTGGATGCTGGTTC |
| TUP | 85,450,943 | 85,450,966 | CGGGTCAGTGAGTAGGCACAACCT |
| TUR | 85,450,889 | 85,450,910 | TCAGTGGGAGGAAAGCTGAAAG |
| TDF | 85,447,331 | 85,447,351 | GTGTTGGGTGCTGAGAAAGTG |
| TDP | 85,447,304 | 85,447,329 | CCAACGCTATACTGCTCTTCATGCCC |
| TDR | 85,447,276 | 85,447,297 | CAGAGAACGGCGGATCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCGTCTATAAGGAGGAGTGC |
| SD | GCAGATGAGCTCCCTACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14014A-G9 | VGB6 | C57BL/6NTac | |
| 14014A-C5 | VGB6 | C57BL/6NTac | |
| 14014A-C10 | VGB6 | C57BL/6NTac | |
| 14014A-G6 | VGB6 | C57BL/6NTac | |
| 14014A-E6 | VGB6 | C57BL/6NTac | |
| 14014A-F2 | VGB6 | C57BL/6NTac | |
