| Gene | Mdp1 |
| Chromosome | 14 |
| Deletion Start | 55,660,371 |
| Deletion End | 55,658,981 |
| Deletion Size | 1,391 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,659,742 | 55,659,762 | AGGCTTGCCTCCAACTCACAG |
| TUP | 55,659,717 | 55,659,741 | AGATCCACATGCCTCTGCCTCCCAA |
| TUR | 55,659,676 | 55,659,694 | AAGGCCGGGCAGTGGATAG |
| TDF | 55,659,068 | 55,659,088 | GCTCTTTGTTCCAGGCGTTAC |
| TDP | 55,659,030 | 55,659,056 | TCCGGGATGGGATGAGTCTTCAAACGT |
| TDR | 55,658,986 | 55,659,005 | GAGTCCAGCTTGGGCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTAGGCCTCCTGTGTAG |
| SD | AAGAAATGCACAGCGTTATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14015A-B5 | VGB6 | C57BL/6NTac | |
| 14015A-C5 | VGB6 | C57BL/6NTac | |
| 14015A-C8 | VGB6 | C57BL/6NTac | |
| 14015A-C12 | VGB6 | C57BL/6NTac | |
| 14015A-D8 | VGB6 | C57BL/6NTac | |
| 14015A-G3 | VGB6 | C57BL/6NTac | |
