| Gene | Ppp1r35 |
| Chromosome | 5 |
| Deletion Start | 137,778,997 |
| Deletion End | 137,780,110 |
| Deletion Size | 1,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,779,452 | 137,779,470 | GCCAGCGGCCAGTTTGATG |
| TUP | 137,779,471 | 137,779,492 | CCTCCAAGGCGGTAGAGGAGCA |
| TUR | 137,779,493 | 137,779,511 | CCGGAACGACTTTCTCAGC |
| TDF | 137,779,638 | 137,779,657 | CGCGCTCTAAGCGACTCTAC |
| TDP | 137,779,663 | 137,779,684 | CCTGGTGAGTCTGCAGGTGCCC |
| TDR | 137,779,685 | 137,779,704 | GCGTTCAGTACCTGCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | AGTCTGACAGGCAAAGACGC |
| SD | TCATCTGAACCTACCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14018A-A5 | VGB6 | C57BL/6NTac | |
| 14018A-B3 | VGB6 | C57BL/6NTac | |
| 14018A-C6 | VGB6 | C57BL/6NTac | |
| 14018A-E8 | VGB6 | C57BL/6NTac | |
| 14018A-G3 | VGB6 | C57BL/6NTac | |
| 14018A-B1 | VGB6 | C57BL/6NTac | |
