14019 2200002J24Rik Status: ES cell colonies screened / QC no positives
| Gene | 2200002J24Rik |
| Chromosome | 7 |
| Deletion Start | 30,699,786 |
| Deletion End | 30,700,401 |
| Deletion Size | 616 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,699,828 | 30,699,846 | TCAGTGCAACCGGCCTATC |
| TUP | 30,699,851 | 30,699,876 | ATTGCCCAGGTCAGAGACAGTCATGC |
| TUR | 30,699,917 | 30,699,937 | AAGCAGAAGGCTGTGATGGAC |
| TDF | 30,700,215 | 30,700,237 | CCATCTTGCTTGTTTGCCTTCAC |
| TDP | 30,700,238 | 30,700,259 | CTCCACAGACCCGGGAAGCCAA |
| TDR | 30,700,264 | 30,700,283 | GCTCGCCTCCAATCTCCAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTTGCTACTGCCAGAGTCAC |
| SD | CATGCCTGTCTTCCCTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
