| Gene | 2310033E01Rik |
| Chromosome | 7 |
| Deletion Start | 24,545,990 |
| Deletion End | 24,541,723 |
| Deletion Size | 4,268 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,545,062 | 24,545,084 | TGACCATATACCCTGTTCTCCAC |
| TUP | 24,545,041 | 24,545,060 | CTCCTTGCTGCAGGGTGCCC |
| TUR | 24,545,017 | 24,545,039 | CCTTTGCACACCTCACAAGTTAG |
| TDF | 24,542,581 | 24,542,598 | TGCAGAGGGCCGAAAGTC |
| TDP | 24,542,554 | 24,542,578 | TGAACACCTTCAAGGCCTGCATGAA |
| TDR | 24,542,507 | 24,542,528 | TGGGAGTCATGGTGGTTGATAC |
PCR Primers
| Primer | Sequence |
| SU | GGGAAAGGAGAAGAGGATGG |
| SD | ATGCATACTGGGATGAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14022B-B9 | VGB6 | C57BL/6NTac | |
| 14022B-D7 | VGB6 | C57BL/6NTac | |
| 14022B-B6 | VGB6 | C57BL/6NTac | |
| 14022C-A9 | VGB6 | C57BL/6NTac | |
| 14022C-A11 | VGB6 | C57BL/6NTac | |
| 14022C-H5 | VGB6 | C57BL/6NTac | |
| 14022C-F8 | VGB6 | C57BL/6NTac | |
| 14022C-G10 | VGB6 | C57BL/6NTac | |
