| Gene | 2410004A20Rik |
| Chromosome | 9 |
| Deletion Start | 73,102,513 |
| Deletion End | 73,104,226 |
| Deletion Size | 1,714 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,102,841 | 73,102,863 | GAGCACATCCCACATCTTGAATC |
| TUP | 73,102,869 | 73,102,895 | TGCACACCCTGATACATGTGAACGTGT |
| TUR | 73,102,918 | 73,102,938 | GGCGGTCCGAATATCCAAATC |
| TDF | 73,104,122 | 73,104,144 | GTTGTTTGGATGGCAGCTATGTC |
| TDP | 73,104,147 | 73,104,170 | AGTTCAGCCTCCAAGGGATGCCTG |
| TDR | 73,104,196 | 73,104,216 | TCCTGGCTCACACCAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | TTGGCCTTGAAGTCAGGTAAC |
| SD | TCGAAACACCAGATGTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14025A-A9 | VGB6 | C57BL/6NTac | |
| 14025A-A11 | VGB6 | C57BL/6NTac | |
| 14025A-C6 | VGB6 | C57BL/6NTac | |
| 14025A-G9 | VGB6 | C57BL/6NTac | |
| 14025A-F12 | VGB6 | C57BL/6NTac | |
