| Gene | 2410015M20Rik |
| Chromosome | 17 |
| Deletion Start | 56,609,665 |
| Deletion End | 56,607,703 |
| Deletion Size | 1,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,609,640 | 56,609,660 | TCGAGTGTGGTCGCTAATGAG |
| TUP | 56,609,604 | 56,609,631 | ACAATCGTGGAGGCCCTGAGAAAGTTCG |
| TUR | 56,609,575 | 56,609,593 | AAGCCACGAGGCAGAGATG |
| TDF | 56,608,277 | 56,608,298 | GCATAAAGCTCTGGGTCCTGTC |
| TDP | 56,608,239 | 56,608,268 | CCCATAAATCAGGCATGGTGTTTGTAGGTC |
| TDR | 56,608,201 | 56,608,223 | CCTTCATTCTCTTGCCTGCACTG |
PCR Primers
| Primer | Sequence |
| SU | CTTAGGCTTGCTTCACCTGG |
| SD | TCCAAACATTACATCTGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14027A-D7 | VGB6 | C57BL/6NTac | |
| 14027B-F6 | VGB6 | C57BL/6NTac | |
| 14027A-C3 | VGB6 | C57BL/6NTac | |
