| Gene | Dctpp1 |
| Chromosome | 7 |
| Deletion Start | 127,260,620 |
| Deletion End | 127,257,036 |
| Deletion Size | 3,585 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,260,547 | 127,260,565 | CCCGCCCGTTCAGATTCAG |
| TUP | 127,260,523 | 127,260,544 | CGGAGCCCACGCTTGAGGACAT |
| TUR | 127,260,502 | 127,260,522 | GGACGGACAAGGACTGCTTAC |
| TDF | 127,257,454 | 127,257,473 | TCTTTCCCACCTGGCTCTAC |
| TDP | 127,257,426 | 127,257,451 | AGGGCCTGGGCGAGTACATTGTTCTT |
| TDR | 127,257,395 | 127,257,414 | TTCAAACCACCACAGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | ACGTCGCATTAGGCTTTCTG |
| SD | GGTCATCAGAGGAGGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14028A-B4 | VGB6 | C57BL/6NTac | |
| 14028A-D2 | VGB6 | C57BL/6NTac | |
| 14028A-H3 | VGB6 | C57BL/6NTac | |
| 14028A-D4 | VGB6 | C57BL/6NTac | |
| 14028A-D11 | VGB6 | C57BL/6NTac | |
| 14028A-F11 | VGB6 | C57BL/6NTac | |
